A New Approach to Quantification /nuclear and Mitochondrial Dna of Biological Traces by Abi Prism® 7700 Sequence Detection Sys
نویسنده
چکیده
We have developed a real-time quantitative PCR assay to measure the concentration of DNA extracted in forensic biological traces. Its rapid and sensitive results were obtained in less than 4 hours. This real-time quantification can be performed on the ABI Prism 7700 Sequence Detector using the fluorogenic probe in a TaqMan assay. We suggest a protocol that will be able to detect and quantitate human DNA and optimize PCR conditions (as the number of cycles) of DNA analysis in forensic caseworks.
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